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KMID : 0545120070170071213
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 7 p.1213 ~ p.1216
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Development of a Monitoring Vector for Leuconostoc mesenteroides Using the Green Fluorescent Protein Gene
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Lee Kwan-Hoon
Kim Han-Geun
Lee Jung-Min
Kim Jeong-Hwan
Park Jeong-Woo
Lee Jong-Hoon
Chung Sung-Kyun
Chung Dae-Kyun
Park Woo-Jung
Kim Joo-Yun
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Abstract
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The vector pCW5 with plasmid pC7, originally isolated in Lactobacillus paraplantarum C7 derived from kimchi, was constructed using a p32 strong promoter, the pC7 replicon, and green fluorescent protein (GFP) as the reporter. The constructed vector was transformed into E. coli and Leuconostoc mesenteroides, and GFP expression detected using a Western blot analysis. GFP fluorescence was recognized in E. coli and Leuconostoc mesenteroides using a confocal microscope. In addition, GFP fluorescence was also clearly detected in several industrially important lactic acid bacteria (LAB), including Lactobacillus bulgaricus, Lactobacillus paraplantarum, and Lactobacillus plantarum. Thus, pCW5 was shown to be effective for Leuconostoc mesenteroides when using GFP as the reporter, and it can also be used as a broad-host-range vector for other lactic acid bacteria
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KEYWORD
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GFP, monitoring system, Leuconostoc mesenteroides, plasmid pC7
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